Urinary excretion of adrenaline and noradrenaline in response to eserine before and after medullo-adrenalectomy in cats.

Removal of the adrenal medulla in cats was performed in two stages under sodium pentobarbital anesthesia. One mg eserine per kg of body weight was in jected subcutaneously before and after medullo-adrenalectomy. Urine of 24-hour period was collected before and after injection of eserine and fluorimetrically analyzed for adrenaline and noradrenaline. The urinary excretion of adrenaline was increased significantly after administration of eserine in both intact and medulloadrenalectomized cats. However, the urinary excretion of adrenaline in response to eserine in medullo-adrenalectomized cats was only 8 per cent of that in intact cats. The urinary excretion of noradrenaline was also increased after injection of eserine. As to the magnitude of increase in noradrenaline excretion there was no definite difference between those before and after medullo-adrenalec tomy.